ADP Lumina

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ADP Lumina 2017-07-19T14:12:33+00:00

ADP Lumina

Procedures:

Add reagent A and incubate at RT for 30 minutes
Add reagent B and measure luminescence

Alternatively, add “1 solution”, incubate for 30 minutes and measure luminescence

Application:

  • Any Kinases
    • Ser/Thr or Tyr protein kinases
    • Lipid and sugar kinases
  • Any ATPases and ion pumps
  • Any other enzymes that generate ADP

Features and Benefits:

  • Quantify ADP in two easy steps
  • No specialized substrate required
  • No need for antibodies, radio-isotopes or beads
  • Superior sensitivity
  • Compatible with all commonly used buffers and reagents
  • Tolerates high concentration of ATP, DTT and Hepes
  • Luminescence (very low compound interference)
  • Homogeneous (ready for 96-, 384-, and 1536- well format)

Standard Curve

Luminescence generated by the assays correlates with ADP concentration.

Application Example 1: Protein Kinase A assayed with ADP Lumina

Application Example 2: PI3 Kinase